Ph.D. Program in Plant Molecular Biology

friday seminar by Mr. Vijendra singh

AKS Lab/ Friday/ June 1, 2018/3.30 pm/ Role of maternal p4-siRNA in seed development
Category: Research

Role of maternal p4-siRNAs in seed development


Vijendra Singh



Polymerase IV dependent small interfering RNAs (p4-siRNAs) are found in plants and are generated in RNA directed DNA methylation (RdDM) pathway of gene silencing. Abundant amount of these p4-siRNAs  has been reported during seed developmental which suggest that they might play important role in plants reproduction. Present study shows severe reproductive defects when the p4-siRNAs mutants are crossed as the female parent, and not when the mutants are used as pollen donors in Brassica rapa. However there was no overt phenotype reported in Arabidopsis. According to another report there is a negative correlation between p4-siRNAs accumulation and AGAMOUS-LIKE (AGL) gene expression level in endosperm of reciprocal crosses of interploidy plants. Thus, p4-siRNAs and AGL62 express in developing seeds and regulates parental genome imbalance in addition to gene expression in interploidy plants. In conclusion,  the outbreeding and interploidy species may  be valuable in understanding the role of RdDM in plant reproduction.
Grover, J.W., Kendall, T., Baten, A., Burgess, D., Freeling, M., King, G.J. andMosher, R.A. (2018) Maternal components of RNA-directed DNA methy-lation are required for seed development in Brassica rapa. Plant J. 94,575–582.
Lu, J., Zhang, C., Baulcombe, D.C. and Chen, J. (2012) Maternal siRNAs as regulators of parental genome imbalance and gene expression in endosperm of Arabidopsis seeds. Proc. Natl Acad. Sci. USA, 109, 5529–5534.