Research Articles 2018 onwards

Friday Seminar by Ms. Kamlesh Kumari

IDG lab/ Friday/ January 19, 2017/3.30 pm/ Virus Induced Gene Silencing (Vigs) Vector Development in Plant Functional Genomics
Category: Research

Virus Induced Gene Silencing (Vigs) Vector Development In Plant Functional Genomics


Virus-induced gene silencing (VIGS) has been used extensively with great potential in plant reverse genetics for the past few years. It is the simplicity, quick and cost effectiveness of the method that makes VIGS instrument as an attractive alternative post transcriptional gene silencing (PTGS) method for studying gene function and high-throughput functional genomics. It is used to identify a loss-of-function phenotype of a desire gene involved in basic cellular functions, metabolic pathways, development biology, plant-microbe interaction, and abiotic stress. VIGS vectors have provided the extreme trust worthy and effectual results to the selective host range. Most of them are functional only in N. benthamiana and other members of the Solanaceae family like tomato. Considering all the evidences it can be concluded that VIGS can strongly be used as a tool for gene function studies but also can be used
for high-throughput functional genomics in plants. In future, it is required to develop wider host ranges of the vector that can successfully influence the effective VIGS in Arabidopsis and rice in particular that will offer future endeavour to the present plant research community.