Sanjay Kapoor

Friday seminar by Ms. Sanskriti ravi


AKS Lab/ May31, 2019/ Friday/ 3.30 PM/  Re-evaluation of transcription factor function in tomato fruit development and ripening with CRISPR/Cas9-mutagenesis.
Category: Research
Posted by: bedineel

 

Re-evaluation of transcription factor function in tomato fruit development and ripening with CRISPR/Cas9-mutagenesis

 

ABSTRACT

Tomato (Solanum lycopersicum) is a model for climacteric fleshy fruit ripening studies. The process of tomato ripening is regulated by multiple transcription factors together with the plant hormone ethylene and their downstream effector genes. Transcription factors (TF) RIN, CNR, TAGL1, AP2a, NOR and FUL1 and FUL2 are major TFs regulating tomato fruit ripening, either by promoting or repressing this process. Their proposed functions were derived from studies of the phenotype of spontaneous mutants or RNAi knock-down lines rather than, actual null mutants. The recent studies used CRISPR/Cas9-mediated mutagenesis to knockout the encoding genes to study TF function in tomato fruit ripening in more detail.