Arun K. Sharma

Friday Seminar by Dr. Zainul Abdeen Khan


IDG Lab/ Friday/ November 17, 2017/ 3.30 pm/Gemnivirus bidirectional promoters
Category: Research
Posted by: bedineel

Abstract

 

Members of the family Geminiviridae, commonly called as geminiviruses, are characterized by having single-stranded, circular DNA genome of 2.5–3.0 kb, which is either monopartite or bipartite. Different geminiviruses can infect either monocotyledonous or dicotyledonous plants, and collectively, these viruses cause a variety of major crop diseases. On the basis of genome organization, insect vector and host range, the family, Geminiviridae is differentiated into nine genera. Begomovirus is the largest genus among geminiviruses having more than 200 species and transmitted by whitefly (Bemisia tabaci) vector. The intergenic region of begomoviruses possess bidirectional promoter. Bidirectional promoters possess the capability of expressing two genes simultaneously, thereby making them superior and functionally more efficient than the normal unidirectional promoter. Insufficient availability of such promoters arises a need and demand for designing and developing functional bidirectional promoters for use in plant genetic engineering. The strong constitutive begomovirus bidirectional promoter developed so far could be very useful for high level constitutive expression of transgenes in a wide variety of plant cells.