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Friday Seminar by Ms. Ridhi Khurana


SK Lab/ Friday/ January10, 2019/3.30 pm/ Intron mediated enhancement and regulation of gene expression
Category: Research
Posted by: bedineel

 

Intron Mediated Enhancement  & Regulation of Gene Expression

 

 

Enhancement of gene expression, tissue-specific and spatial expression are important traits for crop improvement. Regulation of gene expression is attributed to various complex regulatory checkpoints at the transcriptional as well as the post-transcriptional levels. Cis-elements in promoters or upstream regulatory regions of genes, exon shuffling and alternative splicing mechanisms contributed by intronic gene segments regulate which genes are to be expressed in certain tissues and which genes are not to be expressed. Introns have also been shown to not only initiate but also enhance gene expression in mammals, insects, nematodes, yeast and plants by the mechanism of intron mediated enhancement, IME. IME includes any transcript where an intron-containing construct is expressed more than its intron-less counterpart. Reports of IME from different plant and animal groups show the evolutionary power of introns in regulating gene expression. Some of these reports from plant systems will be presented.
 
References

Sieburth, L.E. and Meyerowitz, E.M.(1997). Molecular Dissection of the AGAMOUS Control Region Shows That cis Elements for Spatial Regulation Are Located Intragenically. The Plant Cell 9: 355.

Jeon, J.-S., Lee, S., Jung, K.-H., Jun, S.-H., Kim, C., and An, G.(2000). Tissue-Preferential Expression of a Rice α-Tubulin Gene,OsTubA1, Mediated by the First Intron. Plant Physiology 123: 1005–1014.

Laxa, M., Müller, K., Lange, N., Doering, L., Pruscha, J.T., and Peterhänsel, C.(2016). The 5′UTR Intron of Arabidopsis GGT1 Aminotransferase Enhances Promoter Activity by Recruiting RNA Polymerase II. Plant Physiology 172: 313–327.