Friday Seminar by Dr. Manas Kumar Tripathy


GKP Lab/ Friday/ August 30, 2019/ 3.30 pm/ Functional validation of a small GTP-binding protein PgRab7 in rice for abiotic stress tolerance.
Category: Research
Posted by: bedineel

 

 

Functional validation of a small GTP-binding protein

PgRab7 in rice for abiotic stress tolerance

 

 

Abstract: In this work, we have overexpressed a vesicle trafficking protein, Rab7, from a stress-tolerant plant, Pennisetum glaucum, in a high-yielding but stress-sensitive rice variety Pusa Basmati-1 (PB-1). The transgenic rice plants were tested for tolerance against salinity and drought stress. The transgenic plants showed considerable tolerance at the vegetative stage against both salinity (200 mM NaCl) and drought stress (up to 12 days after withdrawing water). The protection against salt and drought stress may be by regulating Na+ ion homeostasis, as the transgenic plants showed altered expression of multiple transporter genes, including OsNHX1, OsNHX2, OsSOS1, OsVHA, and OsGLRs. In addition, decreased generation and maintenance of lesser reactive oxygen species (ROS), with maintenance of chloroplast grana and photosynthetic machinery was observed. When evaluated for reproductive growth, 89–96% of seed setting was maintained in transgenic plants during drought stress; however, under salt stress, a 33–53% decrease in seed setting was observed. These results indicate that PgRab7 overexpression in rice confers differential tolerance at the seed setting stage during salinity and drought stress and could be a favored target for raising drought-tolerant crops.